Jennifer McCormickEvaluation of CD18/11b Adhesion Molecule on Neutrophil Surface Membrane in Ischemia Reperfusion InjuryRichard BaynosaUniversity of NevadaMicrosurgery2003NVBasic Research Grant

The hypothesis of this project is that hyperbaric oxygen (HBO) inhibits ischemia-reperfusion (IR) induced neutrophil-I CAM adherence by preventing polarization of expressed surface CD 18 molecules. This HBO induced reduced adherence and polarization in neutrophils is due to a systemic increase in plasma nitric oxide (NO). The effect of HBO on systemic NO production is through up regulation of eNOS.

Specific Objectives:

To use a new quantitative method to evaluate the percent of CD 18-polarized and adherent neutrophils formed when reacted with Sham and IR plasma.

To manipulate the percent ofCDl8-polarized and adherent neutrophils formed by treatment with hyperbaric oxygen a nitric oxide scavenger, and a nitric oxide synthase (NOS) inhibitor.

This research should provide insight into the mechanisms of HBO in IR injury, which could significantly impact limb salvage and could have even broader implications in the treatment of IR injury in other organ systems.

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